This is a demonstration of gas chromatograph technique to medical technology students. In this demonstration an ether extract of an unknown anaerobic bacterial culture grown in yeast extract is injected into the gas chromatograph. The spectrum that is produced is given to the students which is used as a "finger print" of the organisms growth on yeast extract and production of volatile and nonvolatile organic acids in liquid culture. This information along with microscopic examination is enough for identification to the Genus - species level (37, 124, 135).
This is a 1000 X microscopic view of Methanobacterium ruminatium a major methane producing bacterium (methanogen) in anaerobic digestion of organic waste material to CH3 and CO2. This is a Gram stain slide preparation showing the Gram positively stained methanogen rods (blue-black) and culture debris as (pink) (98, 124, 229).
503 11 th Ave. SE, Suite 5
Aberdeen, SD 57401
firstname.lastname@example.org Phone: 605 226-3595
Mobile: 605 377-4062
Fax: 605 226-3595