Demonstration Of Gas Chromatograph Technique To Medical Technology Students

Demonstration Of Gas Chromatograph Technique To Medical Technology Students

This is a demonstration of gas chromatograph technique to medical technology students. In this demonstration an ether extract of an unknown anaerobic bacterial culture grown in yeast extract is injected into the gas chromatograph. The spectrum that is produced is given to the students which is used as a "finger print" of the organisms growth on yeast extract and production of volatile and nonvolatile organic acids in liquid culture. This information along with microscopic examination is enough for identification to the Genus - species level (37, 124, 135).


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Methanobacterium ruminatium

Methanobacterium ruminatium

This is a 1000 X microscopic view of Methanobacterium ruminatium a major methane producing bacterium (methanogen) in anaerobic digestion of organic waste material to CH3 and CO2. This is a Gram stain slide preparation showing the Gram positively stained methanogen rods (blue-black) and culture debris as (pink) (98, 124, 229).



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Craig Bremmon
CEBTech Services
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