This technician is injecting a Gas Chromatograph (GC) with 15 ul of an Ether extract of peptone yeast glucose broth that an unknown anaerobic microorganism has been growing in at 37 oC for 12 hours. This is part of the array of tests performed on an unknown anaerobic microbial culture to enable identification.
In addition to GC analysis of a 12 hour old broth extract, microscopic examinations are made of the culture for: morphology, flagellation, spores, motility, Gram Stain reaction (blue for Gram positive, red for Gram negative). Additionally, an array of different carbohydrates can be checked for the culture's ability to metabolize them, change pH, and produce gas (37, 98, 124, 135).
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These are two Gas Chromatographic Spectra of alcohols and volatile fatty acids (left), and methyl derivatives of nonvolatile fatty acids (right). Unknown anaerobic cultures are grown in peptone yeast broth for 12 hours at 37 oC . Then Ether extracts from them are injected onto a GC to search for any of the peaks shown in the above Standard Spectra. The peaks that appear will act as a "Finger print" to aid in culture identification. Along with samples of unknown cultures, an extract of peptone yeast broth with known quantities added of alcohols, volatile, and nonvolatile fatty acids must be submitted for GC analysis at the same time to be used as a control (37, 98, 124, 135).
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