Obligately Anaerobic Colonies (40X) of Peptococcus constellatus in Roll Tube

Obligately Anaerobic Colonies of Peptococcus constellatus (40 X) in Roll Tube

Many types of Obligately Anaerobic Bacteria exhibit an ellipsoidal shape when grown within or under the surface of an agar medium, as shown here by colonies of Peptococcus constellatus grown in an anaerobic roll tube.

Other types or Genera of anaerobic bacteria that can be isolated in anerobic roll tubes include: Fusobacterium, Butyrivibrio, Bacteroides, Leptotrichia, Succinimonas, Succinivibrio, Anaerovibrio, Selenomonas, Vibrio, Eubacterium, Lachnospira, Arachina, Propionibacterium, Actinomyces, Bifidobacterium, Lactobacillus, Clostridium, Treponema, Borrelia, Campylobacter, Acidaminococcus, Gaffkya, Megasphaera, Peptococcus, Peptostreptococcus, Ruminococcus, Sarcina, Streptococcus, and Veillonella (37, 98, 124, 146, 227).

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Clostridium sporogenes (40X) in Roll Tube

Clostridium sporogenes (40 X)

This is a view through a 40 X magnification microscope of a colony of Clostridium sporogenes grown on brain heart infusion agar (BHIA) in an anaerobic Roll Tube. The two black vertical lines are from a marking pen used to make the spiral markings around this Roll Rube for counting purposes. The colony has radiating from it irregularly shaped arms typical of a culture that exhibited motility in early stages of growth. The particles scattered around are fragments of muscle normally present in an infusion medium. Under higher magnification (1000 X) this organism appears as Gram positive medium length rods, motile at early stage of life and some sporulation (37, 98, 124, 227).

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