Anaerobic Inoculation and Colony Selection Apparatus

Anaerobic Inoculation

This a view of the Virginia Polytechnic Institute and State University (Blacksburg, VA) Anaerobic Culture System. It is a complete bench top system designed for handling tube cultures of anaerobes in pre-reduced media. The system provides for handling three tube cultures simultaneously in an oxygen-free CO2 or N2 atmosphere supplied by three gas cannulas under which the tubes are placed when opened.

This riveted aluminum unit is foot-pedal operated providing complete freedom for the operator's hands. By means of foot pedals, the cannulas are moved laterally to a source of flame sterilization. Liquid inoculum is also placed into the tubes via the foot pedals. The tubes inoculated can contain a variety of differential media for identification purposes, or they can contain various antibiotics for antibiotic susceptibility testing.

The center tube position is the motor driven streaker for inoculating roll streak tubes. The streaker can accommodate both 18 and 25 mm tubes (37, 98, 124).


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Inoculation, Transfer and Streaking of a Roll Tube

Inoculation, Transfer and Streaking of a Roll Tube

Pictured is a technician streaking a roll tube anaerobically, slowly drawing the inoculation loop up the inner side of the roll tube (where the agar is) as the roll tube is slowly turned with the motor driven streaker (37, 98, 124).



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