At right of center is a 300 liter computer controlled fermenter, (the vessel supplied by Dairy Crafts Inc. (DCI) of St. Cloud, MN), that has been instrumented with temperature, RPM, dissolved oxygen controlled by agitation or supplemental oxygen addition, antifoam control, pH control by acid/base or ammonia gas addition, automated nutrient addition, and with inlet and exhaust gas monitored by a process mass spectrometer. It is fully computer monitored and controlled (45, 68, 255).
At left is a 28.3 liter (New Brunswick Scientific Co., Inc.) fermenter that has been modified to operate with temperature, pH, dissolved oxygen, and antifoam control. This fermenter is also computer monitored. A variety of microorganisms have been grown in this bioreactor including: E-coli, yeast, Streptomyces, Bacillus, Pseudomonas, Lactobacillus, Azotobacter, Thrichoderma, Fusarium, Klebsiella, Gleotrichium, and Laccaria. Various fermentation modes have been employed including: programmed feeding, batch fermentation, continuous fermentation, fed-batch, plug flow, and as a chemostat (146, 195, 227, 229, 276).
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