Production Fermenters at the National Cancer Institute (NCI), Frederick Cancer Research Facility (FCRF)

Production Fermenters at National Cancer Institute (NCI)

In this view are five fermenters, the two in the background are 120 liter in size, the next two in the center are of the 1,200 liter size, and the largest in the front right corner is 12,000 liter in size. They are all geometrically similar to each other in dimensions. They can be used in studies of scale-up, new media formulations, different run parameters, etc. They have temperature, RPM, sparge air, pH, and dissolved oxygen control. They are each mounted on load cells for continuous weight monitoring. The signal output from sensors and controllers are interfaced with a supervisory computer for data storage, analysis, and graphing.

This group of fermenters has been used for process scale-up and production of a variety of chemotherapeutic antibiotics for NCI including: Alamethicin, Anhydromaggiemycin, Anticapsin, Asparaginase, Anti-Trypanosomal Factor, Clazamycin A, Clazamycin B, Daunorubicin, E. coli pP1T-Mu-a2 IFN, Fredericamycin A48, Gilvocarcin, Julimycin BII, Largomycin, Luteoskyrin, Lyncomycin, Maggiemycin, Pactamycin, PR 1350, PR 1360, Rapamycin, Sangivamycin, and Toyocamycin.

Microorganisms grown in this group of fermenters to produce the above mentioned chemotherapeutic antibiotics were: Trichoderma viride, Streptomyces sp. PD J566, Streptomyces griseoplanus, Erwinia chysanthemi, Pseudomonas fluorescens, Streptomyces violaceorectus, Streptomyces cinereoruber, Streptomyces sp. NSC 82151, Escherichia coli, Streptomyces griseus sp. FCRF 48, Streptomyces arenae, Streptomyces shiodaensis, Streptomyces pluricolorescens, Penicillum islandicum, Streptomyces lincolnesis, Streptomyces sp. PD J566 Var. A21, Streptomyces pactum var. pactum, Streptomyces flocculus var. PR 1350, Streptomyces flocculus var. PR 1360, Streptomyces hygroscopicus, Streptomyces crestomyceticus, and Streptomyces crestomyceticus respectively (14, 45, 68, 73, 98, 136, 146, 226, 227, 229, 230, 126).


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Nutrient Addition Vessel

Nutrient Addition Vessel

This vessel is used for making sterile additions of any one or group of sugars or carbohydrates, a type of complex nitrogen source, or any vegetable oil, like soy oil, to each of the production fermenters as needed (68, 251).



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