Pictured to the right of center is a tangential flow, plate and frame, ultrafiltration (UF) unit that is chemically sterilizable in place. It is used for down stream processing, protein separation and purification, and/or dialysis. This type of UF system utilizes "tangential flow" to prevent clogging. A high flow of pressurized feed (fermentation broth) passes over the UF membrane at a tangent to the membrane surface forcing the permeate to pass through. This action tends to inhibit clogging even with a "transmembrane pressure" differential pressure of 10 psi between the filtrate and retentate (69, 111, 113, 199, 219).
This unit can accommodate UF membrane plates to give up to 15 ft2 of filtration area. It can be fitted with membranes of the following molecular weight cut off (MWCO): 1K, 3K, 5K, 8K, 10K, 30K, 50K, 70K, 100K, 300K, 500K, 1000K, and 0.16um. These membranes can be purchased in the following materials of construction:
The membrane ultra-filtration unit is built by Pall Life Sciences. The supporting equipment i.e. pumps, storage tanks, water purifiers and filters, etc. are provided by other manufacturers.
On the right is a microfiltration unit designed by Membrex called a Pacesetter. This is a unit built for the needs of researchers for a gentle "low shear" action while separating animal or plant cells from the medium or broth they have grown in. It is portable and steam sterilizable in place.
It consists of a one-meter high stainless steel cylinder outer wall, with an internal rotating filter of stainless steel. The action of a rotating internal filter, in combination with the right spacing between the outer wall, and proper RPM, create "Taylor Vortices". Taylor vortices are small currents that flow in a circular motion, in the opposite direction of the internal rotating filter. These Taylor vortices cause a gentle "cleaning effect" on the surface of the rotating filter, preventing clogging. Due to the nature of these Taylor vortices, animal and plant cell walls are not disrupted as much as in other filtration systems (58, 93, 113, 195, 230, 245).
503 11 th Ave. SE, Suite 5
Aberdeen, SD 57401
firstname.lastname@example.org Phone: 605 226-3595
Mobile: 605 377-4062
Fax: 605 226-3595