A 50 Liter Animal Cell Culture Bioreactor Built By ABEC Inc.

A 50 Liter Animal Cell Culture Bioreactor Built By ABEC Inc.

This is a 40 liter Bioreactor designed and built by Associated Bio-Engineers & Consultants Inc. (ABEC). for animal cell culture. Along with the typical control parameters, it is equipped with a specially designed low shear impeller called a "UniShear Impeller" and a rotating filter to retain cells or microcarriers in the bioreactor while removing spent broth as fresh nutrients are added. It can be operated in fed-batch or continuous mode. It has its own clean steam generator which uses plant steam to produce steam from distilled water.

This bioreactor is also computer controlled and has exhaust gas analysis capability by process mass spectrometer. Dissolved oxygen can be controlled by addition of supplemental oxygen along with a proportional amount of carbon dioxide that many animal cell culture systems require for growth. The addition of carbon dioxide also has an effect on the pH of the culture and nitrogen can be proportionally added to strip away excess carbon dioxide (93, 136, 195, 226, 255). ABEC Inc. www.abec.com


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A Three Liter Universal Bioreactor Designed By CEBTech Services

A Three Liter Universal Bioreactor Designed By CEBTech Services

This is a three liter size of a "Universal Bioreactor" design by CEBTech Services. It is capable of being configured for batch or continuous growth of microorganisms, animal cells, or plant cells. It has 14 access ports including: thermowell, dissolved oxygen probe, pH probe, alcohol or cell mass probe, foam probe, sample port, inoculation port, needle injection port, inlet sparge gas, exhaust gas port, cooling water inlet, cooling water outlet, feeding port, and harvest port.

It has three impellers that have 3 arms each with adjustable pitch blades. The agitation system is magnetically coupled and rotates using external and internal rulon bushings. There are two agitator drive ranges, 5 - 200 RPM and 15 - 800 RPM. Temperature control can be by any combination of three methods: silicon heating tape placed around the base, temperature controlled water recycled through heat exchanger coil, and/or heating by external illumination. The bioreactor rests on a platform scale to monitor weight during batch runs or for volume control during continuous runs. The system can be equipped with solid state sensors in the exhaust gas line for measurement of % CO2 and O2 in the exhaust gas.

For research in alcohol production or utilization, a silicon tubing probe can be mounted through the head plate for detecting alcohol concentrations. A constant low flow of air is then sent through fine bore silicone tubing in the probe which is submerged in the culture medium. This air then flows out and through a solid state alcohol sensor, which produces a voltage signal proportional to the alcohol that diffused through the silicone tubing wall and into the air while in the reactor. This voltage is then converted by data logger or controller to concentration of alcohol present in the bioreactor broth.

For research requiring cultivation of microorganisms to high cell density and real time on-line knowledge of cell mass converted to dry cell weight information, a Cerex Corp. MAX Cell Mass Sensor can be mounted through one of the ports to provide this (10, 26, 32, 58, 93, 104, 113, 195, 241, 245, 255).


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Craig Bremmon
CEBTech Services
503 11 th Ave. SE, Suite 5
Aberdeen, SD 57401

bremm001@nvc.net
Phone: 605 226-3595
Mobile: 605 377-4062
Fax: 605 226-3595